Genetics: From Genes To Genomes ISE
Námskeið Erfðafræði. - Höfundar: Leland Hartwell, Leroy Hood, Michael Goldberg, Ann E. Reynolds, Lee Silver
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- Erfðafræði.
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Genetics: From Genes to GenomesAnnað
- Höfundar: Michael Goldberg, Janice Fischer, Leroy Hood, Leland Hartwell
- Útgáfa:8
- Útgáfudagur: 2023-10-03
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- Format:ePub
- ISBN 13: 9781266379956
- Print ISBN: 9781266246678
- ISBN 10: 1266379959
Efnisyfirlit
- Table of Contents and Preface
- Cover Page
- Title Page
- Copyright Page
- About the Author
- Brief Contents
- Contents
- Preface
- A Note from the Authors
- Our Focus—An Integrated Approach
- The Genetic Way of Thinking
- Student-Friendly Features
- Changes in the Eighth Edition
- Guided Tour
- Integrating Genetic Concepts
- Visualizing Genetics
- Solving Genetics Problems
- Acknowledgments
- Connect
- Instructors The Power of Connections
- Students Get Learning that Fits You
- Chapter 1 Introduction
- Mendel’s Principles of Heredity
- 1.1 The Puzzle of Inheritance
- Mendel Devised a New Experimental Approach
- 1.2 Genetic Analysis According to Mendel
- Monohybrid Crosses Reveal the Law of Segregation
- Mendel’s Results Reflect Basic Rules of Probability
- Further Crosses Verify the Law of Segregation
- Dihybrid Crosses Reveal the Law of Independent Assortment
- Mendel’s Laws Predict Probabilities, Not Specific Outcomes
- Mendel’s Genius Was Unappreciated Before 1900
- Recessive Alleles Are Most Often Nonfunctional, While Dominant Alleles Are Usually Functional
- 1.3 Mendelian Inheritance in Humans
- Pedigrees Aid the Study of Hereditary Traits in Human Families
- A Vertical Pattern of Inheritance Indicates a Rare Dominant Trait
- A Horizontal Pattern of Inheritance Indicates a Rare Recessive Trait
- Solved Problems
- Problems
- Chapter 2 Introduction
- Extensions to Mendel’s Laws
- 2.1 Extensions to Mendel for Single-Gene Inheritance
- Dominance Is Not Always Complete
- A Gene May Have More than Two Alleles
- Mutations Are the Source of New Alleles
- Pleiotropy: One Gene May Contribute to Several Characteristics
- Sickle-Cell Disease Illustrates Many Extensions to Mendel’s View of Single-Gene Inheritance
- 2.2 Extensions to Mendel for Two-Gene Inheritance
- Additive Interactions Between Two Genes Can Produce Novel Phenotypes
- Epistasis: One Gene Can Mask the Effects of Another Gene
- Summary: A Variety of Biochemical Pathways Can Produce Any Given Altered Mendelian Ratio
- Incomplete Dominance or Codominance Can Expand Phenotypic Variation
- Locus Heterogeneity: Mutations in Any One of Several Genes May Cause the Same Phenotype
- 2.3 Extensions to Mendel for Complex Trait Inheritance
- The Same Genotype Does Not Always Produce the Same Phenotype
- Mendelian Principles Can Explain Continuous Variation
- 2.4 A Comprehensive Example: Dog Coat Color Genes
- Solved Problems
- Problems
- Chapter 3 Introduction
- Chromosomes and Inheritance
- 3.1 Chromosomes: The Carriers of Genes
- Genes Reside in the Nucleus
- Genes Reside in Chromosomes
- 3.2 Mitosis: Cell Division that Preserves Chromosome Number
- During Interphase, Cells Grow and Replicate Their Chromosomes
- During Mitosis, Sister Chromatids Separate and Two Daughter Nuclei Form
- Regulatory Checkpoints Ensure Correct Chromosome Separation
- 3.3 Meiosis: Cell Divisions that Halve Chromosome Number
- In Meiosis, the Chromosomes Replicate Once but the Nucleus Divides Twice
- During Meiosis I, Homologs Pair, Exchange Parts, and Then Segregate
- During Meiosis II, Sister Chromatids Separate to Produce Haploid Gametes
- Mistakes in Meiosis Produce Defective Gametes
- Meiosis Contributes to Genetic Diversity
- Mitosis and Meiosis: A Comparison
- Mendel’s Laws Correlate with Chromosome Behavior During Meiosis
- Solved Problems
- Problems
- Chapter 4 Introduction
- Sex Chromosomes
- 4.1 Sex Chromosomes and Sex Determination
- In Humans, the SRY Gene on the Y Chromosome Determines Maleness
- Human X and Y Chromosomes Also Contain Genes Unrelated to Sex
- Species Vary Enormously in Sex Determining Mechanisms
- 4.2 Gametogenesis
- Oogenesis in Humans Produces One Ovum from Each Primary Oocyte
- Spermatogenesis in Humans Produces Four Sperm from Each Primary Spermatocyte
- 4.3 Sex Linkage
- The Genes for Sex-Linked Traits Are Located on the X Chromosome
- The Chromosome Theory Integrates Many Aspects of Gene Behavior
- 4.4 Sex-Linked and Sexually Dimorphic Traits in Humans
- In XX Human Females, One X Chromosome Is Inactivated
- Maleness and Male Fertility Are the Only Known Y-Linked Traits in Humans
- Autosomal Genes Contribute to Sexual Dimorphism
- 4.5 Human Intersexuality
- Four Distinct Cell Groups Form Human Sex Organs
- Mutations in Genes that Act After SRY Can Result in Intersexuality
- Solved Problems
- Problems
- Chapter 5 Introduction
- Linkage, Recombination, and Gene Mapping
- 5.1 Gene Linkage and Recombination
- Some Genes on the Same Chromosome Do Not Assort Independently—Instead, They Are Linked
- Testcrosses Simplify the Detection of Linkage
- 5.2 Recombination: A Result of Crossing-Over During Meiosis
- Reciprocal Exchanges Between Homologs Are the Physical Basis of Recombination
- Why Recombination?
- Recombination Frequency Reflects the Distance Between Two Genes
- Recombination Frequencies Between Two Genes Never Exceed 50%
- 5.3 Mapping: Locating Genes Along a Chromosome
- Comparisons of Two-Point Crosses Establish Relative Gene Positions
- Three-Point Crosses Provide More Accurate Mapping
- How Do Genetic Maps Correlate with Physical Reality?
- Multiple-Factor Crosses Help Establish Linkage Groups
- 5.4 The Chi-Square Test and Linkage Analysis
- The Chi-Square Test Evaluates the Significance of Differences Between Predicted and Observed Values
- Applying the Chi-Square Test to Linkage Analysis: An Example
- 5.5 Tetrad Analysis in Fungi
- An Ascus Contains All Four Products of a Single Meiosis
- Tetrads Can Be Characterized as Parental Ditypes (PDs), Nonparental Ditypes (NPDs), or Tetratypes (Ts)
- Recombination Frequencies May Be Determined by Counting Each Tetrad Type
- Ordered Tetrads Help Locate Genes in Relation to the Centromere
- Tetrad Analysis: A Numerical Example
- 5.6 Mitotic Recombination and Genetic Mosaics
- Twin Spots Indicate Mosaicism Caused by Mitotic Recombination
- Mitotic Recombination Can Produce Sectored Yeast Colonies
- Mitotic Recombination Has Significant Consequences
- Solved Problems
- Problems
- Chapter 6 Introduction
- DNA Structure, Replication, and Recombination
- 6.1 Experimental Evidence for DNA as the Genetic Material
- Chemical Studies Locate DNA in Chromosomes
- Bacterial Transformation Implicates DNA as the Genetic Material
- DNA, Not Protein, Contains the Instructions for Virus Propagation
- 6.2 The Watson and Crick Double Helix Model of DNA
- Nucleotides Are the Building Blocks of DNA
- The DNA Helix Consists of Two Antiparallel Chains
- The Double Helix May Assume Alternative Forms
- DNA Structure Is the Foundation of Genetic Function
- 6.3 Genetic Information in Nucleotide Sequence
- Most Genetic Information Is Read from Unwound DNA Chains
- Some Genetic Information Is Accessible Without Unwinding DNA
- In Some Viruses, RNA Is the Repository of Genetic Information
- 6.4 DNA Replication
- Overview: Complementary Base Pairing Ensures Semiconservative Replication
- Experiments with Heavy Nitrogen Verify Semiconservative Replication
- DNA Polymerase Has Strict Operating Requirements
- DNA Replication Is a Tightly Regulated, Complex Process
- The Integrity of Genetic Information Must Be Preserved
- 6.5 Homologous Recombination at the DNA Level
- Tetrad Analysis Illustrates Key Aspects of Recombination
- DNA Molecules Break and Rejoin During Recombination
- Crossing-Over at the Molecular Level: A Model
- DNA Repair of Heteroduplexes Can Result in Gene Conversion
- 6.6 Site-Specific Recombination
- Recombinase Enzymes Catalyze Site-Specific Recombination
- Solved Problems
- Problems
- Chapter 7 Introduction
- Mutation
- 7.1 Mutations: Primary Tools of Genetic Analysis
- Mutations Are Changes in DNA Base Sequences
- Mutations May Be Classified by How They Change DNA
- Spontaneous Mutations Occur at a Low Rate
- Spontaneous Mutations Arise from Random Events
- 7.2 Molecular Mechanisms that Alter DNA Sequence
- Natural Processes Cause Spontaneous Mutations Through DNA Damage
- Mistakes in DNA Replication Cause Spontaneous Mutations
- Mutagens Induce Mutations
- Most Mutagens Are Carcinogens
- 7.3 DNA Repair Mechanisms
- Some DNA Base Damage Can Be Reversed
- Damaged Bases Can Be Removed and Replaced
- Two Important Mechanisms Can Repair Double-Strand Breaks
- Mismatch Repair Corrects Errors in DNA Replication
- Error-Prone Repair Systems Serve as Last Resorts
- Mutations in Genes Encoding DNA Repair Proteins Impact Human Health
- DNA Repair Cannot Be 100% Efficient
- Solved Problems
- Problems
- Chapter 8 Introduction
- Using Mutations to Understand Genes
- 8.1 What Mutations Tell Us About Gene Structure
- Complementation Testing Reveals Whether Two Mutations Are in a Single Gene or in Different Genes
- A Gene Is a Set of Nucleotide Pairs that Can Mutate Independently and Recombine with Each Other
- 8.2 What Mutations Tell Us About Gene Function
- A Gene Contains the Information for Producing a Specific Enzyme: The One Gene, One Enzyme Hypothesis
- Genes Specify the Identity and Order of Amino Acids in Polypeptide Chains
- A Protein’s Amino Acid Sequence Dictates Its Three-Dimensional Structure
- 8.3 What Mutations Tell Us About the Genetic Code
- Triplet Codons of Nucleotides Represent Individual Amino Acids
- A Gene’s Nucleotide Sequence Is Colinear with the Amino Acid Sequence of the Encoded Polypeptide
- Cracking the Code: Which Codons Represent Which Amino Acids?
- The Genetic Code: A Summary
- The Effects of Mutations on Polypeptides Helped Verify the Code
- The Genetic Code Is Almost, but Not Quite, Universal
- 8.4 A Comprehensive Example: Mutations that Affect Vision
- Cells of the Retina Contain Light-Sensitive Proteins
- Mutations in the Rhodopsin Gene Family Affect the Way We See
- Solved Problems
- Problems
- Chapter 9 Introduction
- Gene Expression: The Flow of Information from DNA to RNA to Protein
- 9.1 Transcription: From DNA to RNA
- RNA Polymerase Synthesizes a Single-Stranded RNA Copy of a Gene
- Transcription Initiation Differs in Eukaryotes and Prokaryotes
- In Eukaryotes, RNA Processing After Transcription Produces a Mature mRNA
- 9.2 Translation: From mRNA to Protein
- Transfer RNAs Mediate the Translation of mRNA Codons to Amino Acids
- Polypeptide Synthesis Occurs on Ribosomes
- Ribosomes and Charged tRNAs Collaborate to Translate mRNAs into Polypeptides
- Polypeptides Can Be Modified After Translation
- 9.3 Differences in Gene Expression Between Prokaryotes and Eukaryotes
- In Eukaryotes, the Nuclear Membrane Prevents the Coupling of Transcription and Translation
- Distant Enhancer Sequences and Interactions with Chromatin Influence Eukaryotic Promoters
- Prokaryotes and Eukaryotes Initiate Translation Differently
- Eukaryotic mRNAs Require More Processing than Prokaryotic mRNAs
- 9.4 The Effects of Mutations on Gene Expression and Function
- Mutations in a Gene’s Coding Sequence May Alter the Gene Product
- Mutations Outside the Coding Sequence Can Alter Gene Expression
- Most Mutations that Affect Gene Expression Reduce Gene Function
- Unusual Gain-of-Function Alleles Are Almost Always Dominant
- The Effects of a Mutation Can Be Difficult to Predict
- Mutations in Genes Encoding the Molecules that Implement Expression May Have Global Effects
- Solved Problems
- Problems
- Chapter 10 Introduction
- Digital Analysis of DNA
- 10.1 Fragmenting DNA
- Restriction Enzymes Cut the Genome at Specific Sites
- The Longer the Restriction Enzyme Recognition Site, the Larger the DNA Fragments Produced
- Partial Digestion with a Restriction Enzyme or Mechanical Shearing Generate Overlapping Genomic DNA Fragments
- Gel Electrophoresis Separates DNA Fragments According to Size
- 10.2 Cloning DNA Fragments
- Ligating Inserts to Vectors Produces Recombinant DNA Molecules
- Host Cells Take Up and Amplify Recombinant DNA
- Libraries Are Collections of Cloned Fragments
- 10.3 Sequencing DNA
- Sanger Sequencing Depends on DNA Polymerase
- Sanger Sequencing Generates a Nested Set of DNA Fragments
- DNA Fragment Fluorescence Reveals the Nucleotide Sequence
- 10.4 Sequencing Genomes
- Solved Problems
- Problems
- Chapter 11 Introduction
- Genome Annotation
- 11.1 Finding the Genes in Genomes
- Open Reading Frames (ORFs) Help Locate Protein-Coding Genes
- Whole-Genome Comparisons Distinguish Genomic Elements Conserved by Natural Selection
- The Most Direct Method to Find Genes Is to Locate Transcribed Regions
- 11.2 Genome Architecture and Evolution
- Much of the Human Genome Is Repetitive Intergenic DNA
- The Arrangement of Genes in the Genome Is Not Uniform
- Genomes Evolve
- A Relatively Small Number of Genes Can Produce Enormous Phenotypic Complexity
- Genome Sequence Studies Affirm Evolution from a Common Ancestor
- 11.3 Bioinformatics: Information Technology and Genomes
- Bioinformatics Provides Tools for Visualizing and Analyzing Genomes
- BLAST Searches Automate the Identification of Homologous Sequences
- 11.4 A Comprehensive Example: The Hemoglobin Genes
- Different Hemoglobins Are Expressed at Different Developmental Stages
- The Order of the Hemoglobin Genes in the α and β Clusters Reflects the Timing of Their Expression
- Globin-Related Diseases Result from a Variety of Mutations
- Solved Problems
- Problems
- Chapter 12 Introduction
- Analyzing Genomic Variation
- 12.1 Variation Among Genomes
- Extensive DNA Variation Distinguishes Individuals Within a Species
- Most DNA Polymorphisms Do Not Influence Phenotype
- Genetic Variants Occur in Several Types
- 12.2 Genotyping a Known Disease-Causing Mutation
- The Polymerase Chain Reaction (PCR) Amplifies Defined Regions of a Genome
- PCR Products Are Genotyped by Sequencing or Sizing
- Fetal and Embryonic Cells Can Be Genotyped
- 12.3 Genotyping a Known Disease-Causing Mutation
- Forensic DNA Profiling Examines Multiple SSR Loci
- DNA Microarrays Genotype Millions of SNPs
- 12.4 Positional Cloning
- Linkage Analysis with DNA Markers Gives Disease Genes an Approximate Chromosomal Address
- Positional Cloning Has Several Limitations
- The Lod Score Provides a Statistical Approach to Studying Linkage
- Genetic Diseases Can Display Allelic or Locus Heterogeneity
- 12.5 The Era of Whole-Genome Sequencing
- New Techniques Sequence Millions of Individual DNA Molecules in Parallel
- Disease-Causing Mutations Are Hidden in a Sea of Variation
- Pinpointing a Disease Gene Requires a Combination of Approaches
- The Study of Human Genetics Is an Ongoing Venture
- Solved Problems
- Problems
- Chapter 13 Introduction
- The Eukaryotic Chromosome
- 13.1 Chromosomal DNA and Proteins
- Each Chromosome Is Composed of a Single Long Molecule of DNA
- Chromosomes Contain Histone Proteins and Nonhistone Proteins
- 13.2 Chromosome Structure and Compaction
- The Nucleosome Is the Fundamental Unit of Chromosome Compaction
- Condensins Shape Chromosomes by Extruding Chromatin Loops
- Higher-Order Packaging Condenses Chromosomes Further
- Giemsa Staining Reveals Reproducible Chromosome Banding Patterns
- Fluorescence In Situ Hybridization (FISH) Helps Geneticists Characterize Genomes
- 13.3 Chromosomal Packaging and Gene Expression
- Transcription Requires Changes in Chromatin Structure and Nucleosome Position
- Most Genes in Heterochromatin Regions Are Silenced
- Heterochromatin Can Spread Along a Chromosome and Silence Nearby Euchromatic Genes
- Heterochromatin and Euchromatin Have Different Histone Modifications
- Heterochromatin Formation Inactivates an X Chromosome in Cells of Female Mammals
- 13.4 Replication of Eukaryotic Chromosomes
- Chromosomal DNA Replication Begins at Specific Origins
- New Nucleosomes Must Be Formed During DNA Replication
- Telomeres Protect the Ends of Linear Chromosomes and Allow Their Replication
- 13.5 Chromosome Segregation
- Special DNA Sequences Allow the Formation of Centromeres
- Kinetochores Govern Attachment of Chromosomes to the Spindle
- Cohesin Complexes Hold Sister Chromatids Together
- 13.6 Artificial Chromosomes
- Yeast Artificial Chromosomes (YACs) Help Characterize DNA Elements Required for Chromosome Replication and Transmission
- Yeast Can Survive with a Single Giant Chromosome or Many Minichromosomes
- Synthetic Chromosomes May Help Define Minimal Genomes
- Solved Problems
- Problems
- Chapter 14 Introduction
- Chromosomal Rearrangements
- 14.1 Rearrangements of Chromosomal DNA
- Chromosomal Rearrangements Are Caused by DNA Breakage or Illegitimate Crossing-Over
- A Variety of Methods Can Detect Chromosomal Rearrangements
- 14.2 The Effects of Rearrangements
- Deletions Remove DNA from the Genome
- Duplication Chromosomes Have Extra Copies of Some Genes
- Inversions Reorganize the DNA Sequence of a Chromosome
- Translocations Attach Part of One Chromosome to Another Chromosome
- 14.3 Transposable Genetic Elements
- Transposable Elements Vary in Structure, Copy Number, and Mechanism of Movement
- Nearly Half the Human Genome Consists of Transposable Elements
- Retrotransposons Move via RNA Intermediates
- Transposase Enzymes Catalyze Movement of DNA Transposons
- Genomes Often Contain Defective Copies of Transposable Elements
- Transposable Elements Can Disrupt Genes and Alter Genomes
- Several Mechanisms Limit Transposable Element Movement
- 14.4 Genome Restructuring and Evolution
- Chromosomal Rearrangements Are Important Sources of Genome Variation
- Transposable Elements Can Benefit Their Host
- Duplications Provide Extra Gene Copies that Can Acquire New Functions
- Translocations Contribute to Speciation
- Solved Problems
- Problems
- Chapter 15 Introduction
- Ploidy
- 15.1 Aberrations in Chromosome Number: Aneuploidy
- Autosomal Aneuploidy Is Usually Lethal
- Most Organisms Tolerate Aneuploidy for Sex Chromosomes
- Aneuploidy Arises Through Meiotic Nondisjunction
- Rare Mitotic Nondisjunction or Chromosome Loss Causes Mosaicism
- 15.2 Variation in Number of Chromosome Sets: Euploidy
- Monoploid Plants Are Useful to Plant Breeders
- Triploid Organisms Are Usually Infertile
- Polyploids with an Even Number of Chromosome Sets Can Become New Species
- Allopolyploids Are Hybrids with Complete Chromosome Sets from Two Different Species
- 15.3 Whole-Genome Duplication as a Driver of Evolution
- Solved Problems
- Problems
- Chapter 16 Introduction
- Bacterial Genetics
- 16.1 The Enormous Diversity of Bacteria
- Bacteria Vary in Size and Shape
- Bacteria Have Diverse Metabolisms
- A Small Fraction of Bacteria Are Pathogens
- 16.2 Bacterial Genomes
- Genes Are Tightly Packed in Bacterial Genomes
- Individual E. coli Strains Contain Only a Subset of the E. coli Pangenome
- Bacterial Genomes Contain Transposons
- Plasmids Carry Genes in Addition to Those in the Bacterial Chromosome
- Metagenomics Explores the Collective Genomes of Microbial Communities
- 16.3 Bacteria as Experimental Organisms
- Bacteria Grow in Liquid Cultures or on the Surface of Petri Plates
- E. coli Is a Versatile Model Organism
- Geneticists Identify Mutant Bacteria by the Phenotype of Colonies Under Specific Growth Conditions
- 16.4 Gene Transfer in Bacteria
- In Transformation, the Recipient Takes Up DNA that Alters Its Genotype
- In Conjugation, a Donor Transfers DNA Directly to a Recipient
- In Transduction, a Phage Transfers DNA from a Donor to a Recipient
- Horizontal Gene Transfer Has Significant Evolutionary and Medical Implications
- 16.5 Using Genetics to Study Bacterial Life
- Recombinant Plasmid Libraries Simplify Gene Identification
- Transposons Can Be Used as Gene-Tagging Mutagens
- Gene Targeting Provides a Way to Mutagenize Specific Genes
- 16.6 A Comprehensive Example: How N. gonorrhoeae Became Resistant to Penicillin
- Penicillin Interferes with Synthesis of the Bacterial Cell Wall
- N. gonorrhoeae Become Resistant to Penicillin Through Multiple Mechanisms
- What Should We Do About the Problem of Drug Resistance?
- Solved Problems
- Problems
- Chapter 17 Introduction
- Organellar Inheritance
- 17.1 Mitochondria and Their Genomes
- Mitochondria Produce ATP
- Mitochondrial Genomes Vary Among Species
- Mitochondrial Gene Expression Has Unusual Features
- 17.2 Chloroplasts and Their Genomes
- Chloroplasts Are the Sites of Photosynthesis
- Chloroplast Genomes Are Relatively Uniform
- Scientists Can Produce Transgenic Chloroplasts
- 17.3 The Relationship Between Organellar and Nuclear Genomes
- Nuclear and Organellar Genomes Cooperate with One Another
- Mitochondria and Chloroplasts Originated from Bacteria
- 17.4 Non-Mendelian Inheritance of Mitochondria and Chloroplasts
- In Many Organisms, Organelles and Their DNA Are Inherited from One Parent
- Variants of Organellar Genomes Segregate During Cell Division
- Some Organisms Exhibit Biparental Inheritance of Organellar Genomes
- 17.5 Mutant Mitochondria and Human Disease
- In LHON, Affected People Are Usually Homoplasmic
- In MERRF, Affected Individuals Are Heteroplasmic
- Mitochondrial Mutations May Affect Human Aging
- Oocyte Nuclear Transplantation Can Prevent Transmission of Mitochondrial Disease
- Solved Problems
- Problems
- Chapter 18 Introduction
- Gene Regulation in Prokaryotes
- 18.1 The Elements of Prokaryotic Gene Expression
- RNA Polymerase Is the Key Enzyme for Transcription
- Translation in Prokaryotes Begins Before Transcription Ends
- Regulation of Gene Expression Can Occur at Many Steps
- 18.2 Regulation of Transcription Initiation via DNA-Binding Proteins
- Catabolic and Anabolic Pathways Require Different Types of Regulation
- E. coli’s Utilization of Lactose Provides a Model System of Gene Regulation
- The Operon Theory Explains How a Single Substance Can Regulate Several Clustered Genes
- Genetic Analysis Led Jacob and Monod to the Operon Hypothesis
- Biochemical Experiments Support the Operon Hypothesis
- The lac Operon Is Also Regulated by Positive Control
- Repressor/Effector Interaction Enables Repressible Regulation of Transcription Initiation
- 18.3 RNA-Mediated Mechanisms of Gene Regulation
- Diverse RNA Leader Devices Act in cis to Regulate Gene Expression
- Small RNAs Act in trans to Regulate the Translation or Stability of mRNAs
- Genes Can Also Be Regulated by Antisense RNAs
- 18.4 Discovering and Manipulating Bacterial Gene Regulatory Mechanisms
- lacZ Reporter Genes Help Reveal the Regulation of Other Genes
- lac Operon Regulatory Sequences Help Produce Protein Drugs in Bacteria
- RNA-Seq Is a General Tool for Characterizing Transcriptomes and Their Regulation
- Computer Analysis Reveals Many Aspects of Gene Regulation
- 18.5 A Comprehensive Example: Control of Bioluminescence by Quorum Sensing
- Recombinant V. fischeri Genes Make E. coli Bioluminescent
- LuxI and LuxR Are the Quorum-Sensing Proteins in V. fischeri
- Quorum Sensing Suggests a New Approach to Developing Antibiotics
- Solved Problems
- Problems
- Chapter 19 Introduction
- Gene Regulation in Eukaryotes
- 19.1 Overview of Eukaryotic Gene Regulation
- 19.2 Control of Transcription Initiation Through Enhancers
- Promoters and Enhancers Are the Major cis-Acting Regulatory Elements
- Proteins Act in trans to Control Transcription Initiation
- Enhancers Integrate Cellular Information to Control Gene Transcription
- New Methods Provide Global Views of cis- and trans-Acting Transcriptional Regulators
- Topologically Associating Domains (TADs) Control Enhancer/Promoter Interactions
- 19.3 Regulation After Transcription
- Sequence-Specific RNA-Binding Proteins Can Regulate RNA Splicing
- Several Mechanisms Regulate mRNA Translation
- Ribosome Profiling Measures Translation Efficiency
- Small RNAs Regulate mRNA Stability and Translation
- Posttranslational Modifications Can Change Protein Activity Rapidly
- 19.4 A Comprehensive Example: Sex Determination in Drosophila
- The Number of X Chromosomes Determines Sex in Drosophila
- Sxl Protein Triggers a Cascade of Splicing
- Dsx-F and Dsx-M Proteins Control Development of Somatic Sexual Characteristics
- Solved Problems
- Problems
- Chapter 20 Introduction
- Epigenetics
- 20.1 Transcriptional Regulation Through DNA Methylation
- Methylation at CpG Islands Silences Gene Expression
- Methylation of Insulators Alters TAD Boundaries
- 20.2 Genomic Imprinting
- Sex-Specific DNA Methylation Mediates Imprinting
- Germ-Line Cells Reprogram Methylation Marks
- Human Pedigrees Reveal Imprinting
- Why Did Mammals Evolve Imprinting?
- 20.3 Inheritance of Programmed Gene Repression
- Dividing Cells Retain Memories of Cell Fate
- Dividing Cells Retain Memories of Constitutive Heterochromatin
- Mammalian Cells Retain Memories of Inactivated X Chromosomes
- 20.4 Transgenerational Epigenetic Inheritance
- RNA-Directed DNA Methylation Transmits Information About the On/Off State of Plant Epialleles
- Drosophila piRNAs Transmit Memories of TE Invasion
- 20.5 A Comprehensive Example: Epigenetic Inheritance at the A locus in Mice
- “On”/“Off” Information at AVY Epialleles Is Transmitted Unstably and Can Be Influenced by the Environment
- Are Environmentally Triggered “On”/“Off” States of AVY Inherited?
- Solved Problems
- Problems
- Chapter 21 Introduction
- Manipulating the Genomes of Eukaryotes
- 21.1 Creating Transgenic Organisms
- Scientists Exploit Natural Gene Transfer Mechanisms to Create Transgenic Organisms
- DNA Injection into Pronuclei Generates Transgenic Mice
- Recombinant P Elements Can Transform Drosophila
- Agrobacterium Ti Plasmid Vectors Accomplish Plant Transgenesis
- 21.2 Uses of Transgenic Organisms
- Transgenes Assign Genes to Mutant Phenotypes
- Transgenes Are Key Tools for Analyzing Gene Expression
- Transgenic Cells and Organisms Serve as Protein Factories
- GM Organisms Are Used Widely in Modern Agriculture
- Transgenic Animals Model Human Gain-of-Function Genetic Diseases
- 21.3 Targeted Mutagenesis
- Knockout Mice Have Amorphic Alleles of Specific Genes
- Conditional Knockout Mice Reveal Functions of Essential Genes
- Knockins Introduce Specific Mutations
- CRISPR/Cas9 Allows Targeted Gene Editing in Any Organism
- 21.4 Human Gene Therapy
- Therapeutic Genes Can Be Added to the Genome
- CRISPR/Cas9 Technology Can Repair Mutant Alleles
- Solved Problems
- Problems
- Chapter 22 Introduction
- Genetic Analysis of Development
- 22.1 Model Organisms: Prototypes for Developmental Genetics
- All Living Forms Are Related . . .
- . . . Yet All Species Are Unique
- 22.2 Mutagenesis Screens
- Genetic Screens Identify Genes Required for Specific Developmental Processes
- Primary Mutant Screens Can Miss Key Genes
- Stock Repositories and Genome Sequences Allow Systematic Screening of Mutations
- 22.3 Determining Where and When Genes Act
- Gene Expression Patterns Provide Clues to Developmental Functions
- Mosaic Analysis Can Determine the Focus of Gene Action
- Temperature-Sensitive Alleles Help Determine the Time of Gene Action
- 22.4 Ordering Genes in a Pathway
- The Effects of One Gene on the Expression of Another Can Reveal the Order of Action
- Double Mutant Phenotypes Can Help Determine the Order of Gene Action
- 22.5 A Comprehensive Example: Body Plan Development in Drosophila
- Drosophila Embryos Become Organized into Segments
- Maternal Effect Genes Help Specify Segment Number
- Zygotic Genes Determine Segment Number and Polarity
- Homeotic Genes Specify Parasegment Identity
- Solved Problems
- Problems
- Chapter 23 Introduction
- The Genetics of Cancer
- 23.1 Characteristics of Cancer Cells
- Cancerous Cells Evade Normal Controls on Cell Growth
- Cancer Cells Often Acquire a Potential for Immortality
- Tumors Interact with the Body Abnormally
- Most Cancer Cells Have Unstable Genomes
- 23.2 The Genetic Basis of Cancers
- Cancer Involves the Proliferation of a Clone of Cells
- Cancer Is Generally a Disease of Old Age
- Environmental Mutagens Increase the Likelihood of Cancer
- Some Known Mutations Increase Predisposition to Cancer
- Tumor Genome Sequencing Reveals Multiple Mutations in Cancer Cells
- Feedback Between Cell Proliferation and Genomic Instability Underlies Tumor Progression
- 23.3 How Cell Division Is Normally Controlled
- Growth Factors Initiate Cell Division Through Signal Transduction Cascades
- Cyclins and Cyclin-Dependent Kinases Drive the Cell Cycle
- Cell-Cycle Checkpoints Ensure Genomic Stability
- 23.4 How Mutations Cause Cancer
- Oncogenic Mutations Can Accelerate the Cell Cycle
- Mutations in Tumor-Suppressor Genes Can Release the Cell-Cycle Brakes or Destabilize the Genome
- 23.5 Personalized Cancer Treatment
- Antitumor Drugs Can Target the Products of Oncogenes
- Some Chemotherapies Target Cells that Lack Functional Tumor-Suppressor Genes
- New Cancer Therapies Strengthen the Body’s Immune Surveillance
- Genetic Analysis of Individual Cancers
- Solved Problems
- Problems
- Chapter 24 Introduction
- Variation and Selection in Populations
- 24.1 The Hardy-Weinberg Law: Predicting Genetic Variation in “Ideal” Populations
- Population Geneticists Measure Frequencies that Describe Populations
- The Hardy-Weinberg Law Correlates Allele and Genotype Frequencies
- Many Loci in Human Populations Are Near Hardy-Weinberg Proportions
- 24.2 What Causes Allele Frequencies to Change in Real Populations?
- Hardy-Weinberg Provides a Starting Point for Modeling Actual Populations
- In Finite Populations, Chance Plays a Crucial Role
- Mutations Introduce New Genetic Variation
- Natural Selection Acts on Differences in Fitness to Alter Allele Frequencies
- Balancing Selective Forces Can Maintain Alleles in a Population
- A Comprehensive Example: Human Behavior Can Affect Evolution of Insect Pests
- 24.3 Ancestry and the Evolution of Modern Humans
- Shared Alleles Denote Common Genetic Ancestry
- Modern Humans Originated in Africa
- Modern Humans in Europe and Asia Interbred with Other Hominins
- Solved Problems
- Problems
- Chapter 25 Introduction
- Genetic Analysis of Complex Traits
- 25.1 Heritability: Genetic Versus Environmental Influences on Complex Traits
- Variance Is a Statistical Measure of the Amount of Variation in a Population
- Genetic Variance Can Be Separated from Environmental Variance
- Heritability Is the Proportion of Phenotypic Variance Due to Genetic Variance
- Heritability Studies Examine Phenotypic Variation in Genetic Relatives
- A Trait’s Heritability Determines Its Potential for Evolution
- 25.2 Mapping Quantitative Trait Loci (QTLs)
- Researchers Map QTLs by Analyzing Recombinants Obtained Through Breeding Programs
- Association Mapping Can Identify QTLs in Populations
- QTLs Reflect Population Histories
- Solved Problems
- Problems
- Guidelines for Gene Nomenclature
- General Rules
- Specific Rules for Different Organisms
- Brief Answers to Odd-Numbered Problems
- Chapter 1
- Chapter 2
- Chapter 3
- Chapter 4
- Chapter 5
- Chapter 6
- Chapter 7
- Chapter 8
- Chapter 9
- Chapter 10
- Chapter 11
- Chapter 12
- Chapter 13
- Chapter 14
- Chapter 15
- Chapter 16
- Chapter 17
- Chapter 18
- Chapter 19
- Chapter 20
- Chapter 21
- Chapter 22
- Chapter 23
- Chapter 24
- Chapter 25
- Glossary
- Index
- Solved Problems Graphic Text Alternative (FM)
- Learning Objectives Graphic Text Alternative (FM)
- Genetics and Society Essays Graphic Text Alternative (FM)
- Tools of Genetics Graphic Text Alternative (FM)
- Fast Forward Graphic Text Alternative (FM)
- Feature Figures 10.7 Graphic Text Alternative (FM)
- Table 3.2 Graphic Text Alternative (FM)
- Solved Problems offer Graphic Text Alternative (FM)
- Laptop: Getty Images Graphic Text Alternative (FM)
- iPhone: Getty Images Graphic Text Alternative (Chapter 1)
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- A Pedigree Chart Graphic Text Alternative (Chapter 1)
- A Pedigree Chart Graphic Text Alternative (Chapter 1)
- A Pedigree Chart Graphic Text Alternative (Chapter 1)
- A Pedigree Chart Graphic Text Alternative (Chapter 1)
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- In Generation 1 Graphic Text Alternative (Chapter 2)
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- The Law of Segregation Graphic Text Alternative (Chapter 1)
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- Part A Shows Spindle Fibers Graphic Text Alternative (Chapter 3)
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- Figure A Text Alternative (Chapter 4)
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- A cell with 2 chromosomes Graphic Text Alternative (Chapter 4)
- A cell with 2 chromosomes Graphic Text Alternative (Chapter 4)
- The chromosomes represent Graphic Text Alternative (Chapter 4)
- The drosophila labeled Graphic Text Alternative (Chapter 4)
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- A deceased male and diseased Graphic Text Alternative (Chapter 4)
- Part A shows an unaffected Graphic Text Alternative (Chapter 4)
- Mohan mates with a diseased Graphic Text Alternative (Chapter 4)
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- A deceased male and diseased Graphic Text Alternative (Chapter 4)
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- Huntington disease Graphic Text Alternative (Chapter 7)
- Non-polyQ diseases Graphic Text Alternative (Chapter 7)
- A pedigree chart1 Graphic Text Alternative (Chapter 7)
- A pedigree chart2 Graphic Text Alternative (Chapter 7)
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- Figure A Text Alternative (Chapter 9)
- Figure B Text Alternative (Chapter 9)
- Nucleotide Graphic Text Alternative (Chapter 9)
- base pairing Graphic Text Alternative (Chapter 9)
- tRNA synthetase Graphic Text Alternative (Chapter 9)
- Adermatoglyphia Graphic Text Alternative (Chapter 9)
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- vining legume Graphic Text Alternative (Chapter 9)
- nucleus exists Graphic Text Alternative (Chapter 9)
- nucleus is separated Graphic Text Alternative (Chapter 9)
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- small ribosomal Graphic Text Alternative (Chapter 9)
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- Table 10.1 Graphic Text Alternative (Chapter 10)
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- human genome Graphic Text Alternative (Chapter 11)
- hemoglobin proteins Graphic Text Alternative (Chapter 11)
- Genomic Variation Graphic Text Alternative (Chapter 12)
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- Huntington disease Graphic Text Alternative (Chapter 12)
- Sperm samples Graphic Text Alternative (Chapter 12)
- Microarrays Graphic Text Alternative (Chapter 12)
- PCR Graphic Text Alternative (Chapter 12)
- accompanying figure Graphic Text Alternative (Chapter 12)
- pedigree of a family Graphic Text Alternative (Chapter 12)
- human geneticists Graphic Text Alternative (Chapter 12)
- Lod scores Graphic Text Alternative (Chapter 12)
- The pedigrees Graphic Text Alternative (Chapter 12)
- CFTR Graphic Text Alternative (Chapter 12)
- Figure for Problem 41 Graphic Text Alternative (Chapter 12)
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- A loxP site Graphic Text Alternative (Chapter 21)
- Poly-A addition site Graphic Text Alternative (Chapter 21)
- Cas9 protein Graphic Text Alternative (Chapter 21)
- BbsI recognition site Graphic Text Alternative (Chapter 21)
- Capital letters GraphicCapital letters Graphic Text Alternative (Chapter 21)
- Sickle-cell disease Graphic Text Alternative (Chapter 21)
- Figure 22.1 Text Alternative (Chapter 22)
- Figure 22.2b, c, d Text Alternative (Chapter 22)
- Figure 22.3 Text Alternative (Chapter 22)
- Figure 22.4 Text Alternative (Chapter 22)
- Figure 22.5 Text Alternative (Chapter 22)
- Figure 22.6 Text Alternative (Chapter 22)
- Figure 22.7 Text Alternative (Chapter 22)
- Figure 22.9a Text Alternative (Chapter 22)
- Figure 22.10 Text Alternative (Chapter 22)
- Figure 22.11 Text Alternative (Chapter 22)
- Figure 22.12 Text Alternative (Chapter 22)
- Figure 22.13 Text Alternative (Chapter 22)
- Figure 22.14 Text Alternative (Chapter 22)
- Figure 22.15b, c Text Alternative (Chapter 22)
- Figure 22.16 Text Alternative (Chapter 22)
- Figure 22.17 Text Alternative (Chapter 22)
- Figure 22.19 Text Alternative (Chapter 22)
- Figure 22.21 Text Alternative (Chapter 22)
- Figure 22.22 Text Alternative (Chapter 22)
- Figure 22.23 Text Alternative (Chapter 22)
- Figure 22.24b Text Alternative (Chapter 22)
- Figure 22.25 Text Alternative (Chapter 22)
- Figure 22.26a Text Alternative (Chapter 22)
- Figure 22.27 Text Alternative (Chapter 22)
- Figure 22.28 Text Alternative (Chapter 22)
- Figure 22.29 Text Alternative (Chapter 22)
- Figure 22.31 Text Alternative (Chapter 22)
- Fertilization Graphic Text Alternative (Chapter 22)
- Restrictive temperature Graphic Text Alternative (Chapter 22)
- Wild type Graphic Text Alternative (Chapter 22)
- Hunchback and Krüppel protein Graphic Text Alternative (Chapter 22)
- PcG mutant Graphic Text Alternative (Chapter 22)
- Arabidopsis thaliana Graphic Text Alternative (Chapter 22)
- Scientists tested the flower Graphic Text Alternative (Chapter 22)
- Figure 23.1 Text Alternative (Chapter 23)
- Figure 23.2 Text Alternative (Chapter 23)
- Figure 23.4a Text Alternative (Chapter 23)
- Figure 23.5 Text Alternative (Chapter 23)
- Figure 23.6 Text Alternative (Chapter 23)
- Figure 23.7 Text Alternative (Chapter 23)
- Figure 23.8 Text Alternative (Chapter 23)
- Figure 23.10 Text Alternative (Chapter 23)
- Figure 23.11 Text Alternative (Chapter 23)
- Figure 23.12 Text Alternative (Chapter 23)
- Figure 23.13 Text Alternative (Chapter 23)
- Figure 23.14 Text Alternative (Chapter 23)
- Figure 23.15 Text Alternative (Chapter 23)
- Figure 23.16 Text Alternative (Chapter 23)
- Figure 23.17 Text Alternative (Chapter 23)
- Figure 23.19 Text Alternative (Chapter 23)
- Figure 23.20 Text Alternative (Chapter 23)
- Figure 23.21 Text Alternative (Chapter 23)
- Figure 23.22a Text Alternative (Chapter 23)
- Figure 23.23b, c Text Alternative (Chapter 23)
- Figure 23.24 Text Alternative (Chapter 23)
- Figure 23.25 Text Alternative (Chapter 23)
- Figure 23.27 Text Alternative (Chapter 23)
- Figure 23.28 Text Alternative (Chapter 23)
- Figure 23.30 Text Alternative (Chapter 23)
- Experiment 1 and experiment 2 Graphic Text Alternative (Chapter 23)
- A carcinogenic compound Graphic Text Alternative (Chapter 23)
- Growth factor receptor Graphic Text Alternative (Chapter 23)
- Hereditary breast and ovarian cancer Graphic Text Alternative (Chapter 23)
- For a tumor-suppressor gene Graphic Text Alternative (Chapter 23)
- Figure 24.1 Text Alternative (Chapter 24)
- Figure 24.2 Text Alternative (Chapter 24)
- Figure 24.3 Text Alternative (Chapter 24)
- Figure 24.4 Text Alternative (Chapter 24)
- Figure 24.5 Text Alternative (Chapter 24)
- Figure 24.6 Text Alternative (Chapter 24)
- Figure 24.7 Text Alternative (Chapter 24)
- Figure 24.8 Text Alternative (Chapter 24)
- Figure 24.10 Text Alternative (Chapter 24)
- Figure 24.11 Text Alternative (Chapter 24)
- Figure 24.12 Text Alternative (Chapter 24)
- Figure 24.13 Text Alternative (Chapter 24)
- Figure 24.14 Text Alternative (Chapter 24)
- Figure 24.15 Text Alternative (Chapter 24)
- Figure 24.16b, c Text Alternative (Chapter 24)
- Figure 24.17 Text Alternative (Chapter 24)
- Figure 24.18 Text Alternative (Chapter 24)
- Figure 24.21 Text Alternative (Chapter 24)
- Figure 24.22 Text Alternative (Chapter 24)
- Generation Graphic Text Alternative (Chapter 24)
- DNA samples analyzed Graphic Text Alternative (Chapter 24)
- The frequencies of alleles Graphic Text Alternative (Chapter 24)
- A cladogram Graphic Text Alternative (Chapter 24)
- Figure 25.2 Text Alternative (Chapter 25)
- Figure 25.3b Text Alternative (Chapter 25)
- Figure 25.4 Text Alternative (Chapter 25)
- Figure 25.5 Text Alternative (Chapter 25)
- Figure 25.6b, c, d Text Alternative (Chapter 25)
- Figure 25.7 Text Alternative (Chapter 25)
- Figure 25.8 Text Alternative (Chapter 25)
- Figure 25.9 Text Alternative (Chapter 25)
- Figure 25.10 Text Alternative (Chapter 25)
- Figure 25.12 Text Alternative (Chapter 25)
- Figure 25.13 Text Alternative (Chapter 25)
- Figure 25.14 Text Alternative (Chapter 25)
- Figure 25.15 Text Alternative (Chapter 25)
- Figure 25.16 Text Alternative (Chapter 25)
- Figure 25.17 Text Alternative (Chapter 25)
- Figure 25.18 Text Alternative (Chapter 25)
- Figure 25.19 Text Alternative (Chapter 25)
- Figure A Text Alternative (Chapter 25)
- Figure 25.20 Text Alternative (Chapter 25)
- Figure 25.22 Text Alternative (Chapter 25)
- Parental generation Graphic Text Alternative (Chapter 25)
- Systemic lupus erythematosus Graphic Text Alternative (Chapter 25)
- In GWAS analysis Graphic Text Alternative (Chapter 25)
- Mitosis Graphic Text Alternative (Answers)
- Meiosis II Graphic Text Alternative (Answers)
- Metaphase I Graphic Text Alternative (Answers)
- Meiosis metaphase Graphic Text Alternative (Answers)
- NDJ occurred in male meiosis Graphic Text Alternative (Answers)
- Distal Graphic Text Alternative (Answers)
- White flowers Graphic Text Alternative (Answers)
- The parental genotype Graphic Text Alternative (Answers)
- Tetrad analysis in plants Graphic Text Alternative (Answers)
- Trp− phenotype Graphic Text Alternative (Answers)
- Mitotic recombination Graphic Text Alternative (Answers)
- Smc homozygotes Graphic Text Alternative (Answers)
- Homologous chromosomes Graphic Text Alternative (Answers)
- Gene conversion Graphic Text Alternative (Answers)
- Homologous recombination Graphic Text Alternative (Answers)
- Recombinase action Graphic Text Alternative (Answers)
- DNA rearrangements Graphic Text Alternative (Answers)
- The colored boxes in the table represent Graphic Text Alternative (Answers)
- The first table is a complementation test Graphic Text Alternative (Answers)
- X-linked genes cause hemophilia Graphic Text Alternative (Answers)
- Glutamine Graphic Text Alternative (Answers)
- Embryo surface Graphic Text Alternative (Answers)
- The in-frame stop codon Graphic Text Alternative (Answers)
- Vector Graphic Text Alternative (Answers)
- Half-sibling Graphic Text Alternative (Answers)
- Haploidentical Paternal Graphic Text Alternative (Answers)
- 10,000-fold compaction Graphic Text Alternative (Answers)
- Crosslinked chromatin Graphic Text Alternative (Answers)
- Sister chromatids Graphic Text Alternative (Answers)
- Giant fused chromosome Graphic Text Alternative (Answers)
- Translocation Graphic Text Alternative (Answers)
- Meiotic nondisjunction Graphic Text Alternative (Answers)
- Gametes Graphic Text Alternative (Answers)
- The gene order is pyr trp cys Graphic Text Alternative (Answers)
- Genomic DNA Graphic Text Alternative (Answers)
- The GFP sequences are cDNA Graphic Text Alternative (Answers)
- In-frame fusion Graphic Text Alternative (Answers)
- Specificity for binding Graphic Text Alternative (Answers)
- Intergenic regions Graphic Text Alternative (Answers)
- Resting cells Graphic Text Alternative (Answers)
- Bacterial promoter Graphic Text Alternative (Answers)
- Percentage AKR allele Graphic Text Alternative (Answers)
- Clone gene for fish antifreeze Graphic Text Alternative (Answers)
- Construct for gene targeting Graphic Text Alternative (Answers)
- Knockin construct Graphic Text Alternative (Answers)
- Gene X genomic DNA Graphic Text Alternative (Answers)
- Target cleaved Graphic Text Alternative (Answers)
- All the progeny Graphic Text Alternative (Answers)
- sgRNA Graphic Text Alternative (Answers)
- FLP-induced crossover Graphic Text Alternative (Answers)
- Mosaic ommatidia Graphic Text Alternative (Answers)
- X chromosome homologs Graphic Text Alternative (Answers)
- Mutation Graphic Text Alternative (Answers)
- Ancestral allele Graphic Text Alternative (Answers)
- To isolate DNA corresponding Graphic Text Alternative (Answers)
UM RAFBÆKUR Á HEIMKAUP.IS
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